Introduction: A chest tube insertion is a surgical procedure to remove the air, blood, pus, lymph, and fluid from the pleural space by inserting a hollow, flexible drainage tube through the side of the chest in the pleural space. A chest tube is a widespread therapeutic intervention for patients admitted to medical and surgical care areas. It is associated with significant morbidity and mortality. Objectives: To assess the knowledge of staff nurses regarding the care of the patient with chest tube drainage, To evaluate the effectiveness of the structured interventional program on knowledge regarding care of the patient with chest tube drainage among staff nurses, to find out the association between pre-test scores of knowledge regarding care of the patient with chest tube drainage with selected socio-demographic variables. Methods: A quantitative one group pre-test and post-test design were conducted at IGMC and Hospital Shimla, Himachal Pradesh. A total of 40 staff nurses were selected by convenience sampling technique. The tool used for data collection was a structured knowledge questionnaire. A structured interventional program on care of patients with chest tube drainage with the use of ppt was administered. Results: The majority of the subjects 19(47.5%) fall in the age group between 26-30 years. With respect to education, 19(47.5) have done B.Sc nursing. 22 (55.0%) of the staff nurses had experienced between 2-3 years, and 34(85.0) of them belong to the Hindu religion. The majority had 20 (70%) average knowledge, 16 (40%) of staff nurses had poor knowledge and 4(10%) of them had good knowledge whereas after intervention majority 28(70%) of the subjects have good knowledge, 12(30%) of subjects has average knowledge and none of the subjects has poor knowledge regarding care of patients with chest tube drainage. The mean knowledge score in the pre-test was (16.6 ±7.98), in the post-test was (31.65±6.12) which indicates there is an increase in knowledge of the subjects. Conclusion: The study concluded that a structured interventional program on the care of the patient with chest tube drainage was found to be effective in increasing the knowledge of staff nurses. Staff nurses had a significant gain in knowledge regarding the care of patients with chest tube drainage.