The present study is related to measurement of professionalism of college teachers and to find out whether is there any significant difference towards professionalism of college teachers as far as the gender of the teacher , teachers working in various faculties and teaching experience are concerned. In this study, a Likert type five point attitude scale of twenty statements was developed and used by the researcher. Fifty six teachers working in different stream of Rajkot (Gujarat) were selected as the sample, among them 28 were male teachers and 28 were female teachers. Analysis and scoring of the study were done by t-Test and f-Test. The findings revealed that Gender, faculties i.e. Arts, Science, Commerce & Education and teaching experience had significant effect upon teachers attitude towards professionalism