O-Toluidine is a very specific reagent used widely in various Laboratory. It finds place in forensic medicines laboratory where human blood stains on various objects are tested and used for crime detection. Besides these O-Toluidine is utilized in synthesis of many useful organic compounds. A valuable report has been published on germicidal action of O-Toluidine. Thus selection of O-Toluidine for mechanistic studies seems interesting and inviting as well