India is one of the largest and most populated countries in the world, with over one billion inhabitants of this number, it’s estimated that around 2.5 million people are currently living with HIV. One group pre-test and post-test design was used in this study. A purposive sampling technique was used to select 60 Jr. College students. Based on the objective collected data was analyzed by using descriptive and inferential statistics like mean, median, standard deviation, paired t-test, and chi-square test. An analysis finding depicts that the corresponding p<0.05, so the null hypothesis is rejected. The change in the post-test knowledge score (23.76) of Jr. College students is significantly higher than the pre-test score (15.73). The structured teaching program is proven an effective method to improve the knowledge of Jr. College students regarding HIV-AIDS. The study also had a significant association between knowledge score and Age and other variables are not significant.