This dissertation explores the impact of homophobia on LGBTQIA+ individuals, focusing on lesbian, gay, bisexual, transgender, queer, intersex, asexual, and other gender identities. Homophobia, defined as an irrational fear or hatred of LGBTQIA+ individuals, can manifest in various forms such as discrimination, bullying, harassment, violence, and denial of basic human rights. A sample of 100 college students was used to measure homophobia levels by the Italian version of the Homophobia Scale Questionnaire and ANOVA was conducted to examine if there were significant differences in the mean of the response variable across different treatment groups. The results indicated that there is a substantial difference in the mean of TREATMENT across the various treatment groups, with a significant F ratio. The rejection of the null hypothesis suggests that the impact of homophobia on the lives of LGBTQIA+ individuals varies significantly depending on the level of homophobia experienced. This study contributes to the existing literature by shedding light on the detrimental effects of homophobia on the lives of LGBTQIA+ individuals. The findings emphasize the urgent need for creating awareness, fostering inclusivity, and implementing measures to combat homophobia in order to ensure the well-being and equal rights of LGBTQIA+ individuals in society.