Blood group system was disclosed by Karl Landsteiner, the ABO blood group system is the most vital blood group system in Transfusion Medicine. The blood group systems are also relevant in population genetic studies, researching population migration patterns as well as in solving certain medico-legal issues. This study will be carried out with an objective to provide data regarding gene frequency and distribution of ABO and RH blood groups in the population of Bhopal, Madhya Pradesh, India. Blood samples will be collected from willing individuals of Bhopal (M.P.) 180 blood samples are required for this study. Before blood sample collection we take written consent of subjects and short questionnaire based on such as an address, age, any type of diseases, medical history and sex. After blood sample collection the samples will analyze for blood group ABO and Rh factor by slide agglutination method by using Anti-A, Anti- B, Anti-D per ABO blood grouping.