The segmentation algorithms vary for the types of medical images such as MRI, CT, US, etc. The current study work can further be extended to develop a GUI tool based approach for separating the ROI. Additionally, a new technique of separating ROI from the original image that will be applicable for all type of medical images can be evolved. Separated ROI can be stored with min, xmax, ymin and ymax value so that at the end of embedding process before transmitting watermarked image, the segmented ROI can be attached with watermarked image. Any medical image watermarking approach will be suitable, if we segment the ROI from the medical image with the four values, then embedding of the watermark can be done on whole medical image, in this paperwork on a different scan like ctscan ,brain scan etc. our results significant high than other.