Mental depression is one of the common chronic illnesses that affect the mood, thought, physical health and behavior of an individual. India is a rich source of medicinal plants used therapeutically to treat various disorders including depression. This study was undertaken to evaluate the anti-depressant effect of acute and chronic administration of fresh fruit juice of Malus domestica in experimental animal models. We used the fresh fruit juice extract of Malus domestica (1 ml/kg and 2 ml/kg), the standard drug used was Imipramine (10 mg/kg) and the vehicle was 1% tween 80 (1 ml/kg), orally. Four groups of animals were used and each group had six animals. In the acute study drugs/vehicles were administered 60 min prior to the experiments. In the chronic study drugs/vehicles were administered for 10 days and the last dose was given on the 10th day, 60 minutes prior to experiment. Forced Swim Test and Tail Suspension Test were used for testing antidepressant activity and parameters estimated includes estimation of biochemical parameter (mono amino oxidase). Data was analysed using one-way ANOVA with drug treatment as the independent factor. Post-hoc comparisons were performed using Dunnett’s test. In acute and chronic forced swim test as well as tail suspension test, duration of immobility was significantly reduced in the FFJMD treated group but more beneficial effect found in chronic administration in both models and decrease in a biochemical parameter such as mono amino oxidase when compared with depressive control. The antidepressant activity of 2 ml/kg was comparable to that of Imipramine 10 mg/kg. The present study suggests that fresh fruit juice of Malus domestica has more beneficial antidepressant activity in chronic administration of 2 ml/kg. It would be advisable to encourage consumption of Malus domestica extract in patients with depression because of its nutritional and functional properties. From the present data, it concludes that fresh fruit juice of Malus domestica possesses significant anti-depressant activity.