Objective: To evaluate the anti-depressant effect of acute and chronic administration of fresh fruit juice of Malus domestica in experimental animal models. Methods: Anti-depressant activity of fresh fruit juice of Malus domestica was investigated in experimental animal models. Two doses 0.5ml and 1ml of FFJMD (oral route) was subjected for the evaluation as acute (1day) and chronic treatment (10days). Imipramine (10mg/kg oral) was used as standard in all the models of animals and parameters estimated includes estimation of biochemical parameter (mono amino oxidase). Results: Both the lower (0.5ml) and higher dose (1ml) of Malus domestica fresh fruit juice showed dose dependent significant decrease in depression. In acute and chronic forced swim test as well as acute tail suspension test, duration of immobility was significantly reduced in the FFJMD 1 ml and 0.5 ml treated group but the effectiveness was found more in FFJMD 1 ml. In hole board test there is increase in activity with FFJMD 0.5 ml and 1 ml treated groups and increase in biochemical parameter such as mono amino oxidase when compared with depressive control. The antidepressant activity of 1 ml was comparable to that of Imipramine 10 mg/kg. Conclusion:The present study suggests that fresh fruit juice of Malus domestica has antidepressant activity in both the doses but more beneficial effect was found in chronic administration at 1 ml. It would be advisable to encourage consumption of Malus domestica extract in patients with depression because of its nutritional and functional properties.