The combination of the two molecules (Bromacil & Terbuthylazine) is being used effectively as a weedicide worldwide due to its unique way of acting to kill the weeds. Since these two molecules (Bromacil & Terbuthylazine) has very good penetrating power in the earth crust, it is very important to get analysed for its identification and quantification to determine the shelf life in the substrate by detecting the minimum residue level (MRL) in the existing substrate. A simple HPLC chromatographic method has been developed and subsequently validated for the combination pesticide (Bromacil + Terbuthylazine) separation and quantification. These molecules were separated through a mobile phase consisting of the mixture of acetonitrile and water ratio of 80:20 v/v. The separation was achieved through the Qualisil BDS C18 (250 x 4, 5μ) column with the flow rate as 1.0 ml/min with the detection at 220 nm. These method parameters were loaded in the Shimadzu HPLC (model: LC-2030). The LC solution Shimadzu software was used for all the calculations in this analytical method validation analysis. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of these molecules interims of validation parameters viz., separation, system suitability, System Precision and linearity in a simple HPLC analysis.