A simple reverse phase liquid chromatographic method has been developed and subsequently validated for the Flonicamid are a fungicide molecule; which is applicable for the treatment of the vegetable and fruits fungal decease. These Flonicamid molecules were separated through a mobile phase consisting of the mixture of acetonitrile, methanol, and water in the ration of 10:30:60. All these solvents are HPLC grade. Column: Qualis BDS C18 (250 x 4, 5μ); Flow rate: 1.0 ml/min; Detector: UV-Vis. Absorption (λ) at 230 nm of Shimadzu HPLC (model: LC-2030). The LC solution software was used for the analytical method, data integrations, and calculations in this analysis. There are two molecules were analyzed for separation and quantification. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of these molecules interims of validation parameters viz., separation, system suitability, System Precision and linearity in a simple HPLC analysis.