A simple reverse phase liquid chromatographic method has been developed and subsequently validated for the Mesotrione + S -Metolachlor + Benoxacor combination pesticide molecule. These molecules mixtures have been used as an effective weedicide, microbial control effectively in the presence of a chemical. The combination products Mesotrione + S -Metolachlor + Benoxacor is being used as a pesticide to manage at a time soil, crop plants, and weed management effectively. Such an important application of these combination products has fairly few analytical methods. These Mesotrione 4% + S -Metolachlor 40% + Benoxacor 2% molecules were separated through a mobile phase consisting of the mixture of acetonitrile and water in the ratio of 80:20 (v/v). The Column used in this separation is Shimadzu BDS C18; 250 mm length; 4.6 mm diameter with 5μ particle size dimensioned column; Flow rate: 1.0 ml/min; Detector: UV-Vis. Absorption (λ) at 230 nm of Shimadzu HPLC (model: LC-2030). The LC solution software was used for the analytical method, data integrations, and calculations in this analysis. There are two molecules were analyzed for separation and quantification. The results of the study showed that the proposed HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of these molecules interims of validation parameters viz., separation, system suitability, System Precision and linearity in a simple HPLC analysis.