The aim of the study was to determine the strong antimicrobial and antifungal activity from the extracts of Peganum Harmala plant species by solvent extraction procedure. P. harmala seeds extract contains several important alkaloids such an important Oxamide compound was used for this study. Extracted product was isolated, purified and identified by Thin Layer Chromatography, UV-VIS Spectroscopy, FT-IR Spectroscopy and 1H-NMR, 13C-NMR Spectroscopy. Finally, the isolated compound Oxamide activity was checked and proved through the Candida albicans, Klebsiella pneumonia and Pseudomonas aeruginosa with MIC studies.