Rice is a major food crop in India which is affected by both biotic and abiotic stresses. Rice is infected by many diseases that are caused by fungi, bacteria, and virus. Among them, Rhizoctonia solani is an important pathogen which causes a major yield loss in rice. The application of chemicals hazardous to humans causing environmental pollution. Hence, in order to reduce these problems, there is a need for sustainable agriculture using natural resources to compact the diseases. In the present study, The PGPR Pseudomonas were collected from different places and were evaluated against the Rhizoctonia solani under in vitro conditions. Among the five isolates, Pf1 showed the highest reduction percentage against the pathogen.