Urinary tract infections are common during pregnancy and the most common causative organisms is Escherichia coli. Pregnant women are at increased risk for UTIs. Beginning in week 6 and peaking during weeks 22to 24, approximately 90 percent of pregnant women developed ureteral dilation ,which will remain until delivery increase bladder volume and decrease bladder tone ,contribute to increased urinary stasis and ureterovesical reflux, the physiologic increase in plasma volume during pregnancy decrease urine concentration. Up to 70 percent of pregnant women develop glycosuria, which encourages bacterial growth in the urine. Increase in urine progestins and estrogens may lead to a decreased ability of the lower urinary tract to resist invading bacteria allowing some strains of bacteria to selectively grow. These factors may all contribute to the development of Escherichia coli during pregnancy. The purpose of this study was to evaluate the prevalence and their antibiogram profile of uropathogenic Escherichia coli (UPEC) in and around Dhanbad. Materials and methods: A total of 641 urine samples were collected from the suspected patients of UTI. The samples were cultured on MacConkey agar for isolation and identification. Antibiotic susceptibility test was done by disc diffusion method. Female pregnant patients of different age groups were included for this study. Result: 29.48% urinary isolates were identified as Escherichia coli. 51.32% UPEC isolates were sensitive to Nitrofurantoin and Piperacillin/Tazobactum. 25.38% isolates were susceptible to Levofloxacin and Amikacin followed by Cefotaxime (12.16%). Conclusion: Prevalence of Escherichia coli among urinary isolates was high in our study. Antibiogram profile of these isolates varies to different antibiotics in terms of their susceptibility pattern. Continuous surveillance of Antibiogram profile of UPEC isolate is mandatory because it vary significantly.