Aqueous extract of Trachyspermum Ammi seeds (AETAS) was evaluated for its hepatoprotective activities in rats. The plant extract (200 and 400mg/kg, p.o.) showed a remarkable hepatoprotective and antioxidant activity against paracetamol-induced hepatotoxicity is judged from the serum marker enzymes and antioxidant level. Paracetamol - induced significant rise in AST, ALT, ALP, Total bilirubin with a reduction of total protein, albumin, SOD, Catalase, GSH and marked rise in LPO level. Treatment of rats with different doses of plant extracts (200 and 400mg/kg) significantly altered serum marker enzymes, antioxidant levels to near normal against paracetamol treated rats. The activity of the extracts was comparable to the standard drug silymarin (100mg/kg, p.o). Results indicate the hepatoprotective properties of (AETAS) against paracetamol-induced hepatotoxicity in rats.