fungal species are useful for medical and agriculture purpose as a bio- control agent. In aquatic environments, temperature, salinity and organic pollution are the important factors.The objective of the current project was to investigate the possibilities of controlling the mosquito population using fungal cultures. to investigate the possibilities of controlling the mosquito population using fungal cultures. In the view of rampant increase in the incidence of dengue fever transmitted through mosquitoes it is absolutely essential to control the mosquito population. There are different species of mosquitoes of which dengue virus is transmitted by the Aedes aegypti, found in habitats like stagnant clear waters, backyards which are common in urban areas. In the present work water samples were collected from in and around Mumbai, the water samples were examined for its nutritional status by analyzing them physically, chemically and biologically.The fungal cultures obtained from water samples were predominantly Aspergillus niger, Aspergillus terreus, and Penicillium, Trichoderma, Curvularia, Sprolegmia and Achlya.. As per the results it was observed that the Curvularia was the most effective on the larvae as the mortality rate was 100%. . The death of the larvae could be possibly due to the organic acids produced by the fungi. Microscopic observation of the larvae shows the growth of the fungi on larvae ultimately killing it. This is the most effective bio-control.