Soil and water pollution is becoming one of major burden in modern Indian society due to industrialization. Though there are many methods to remove the heavy metal from soil and water pollution but biosorption is one of the best scientific method to remove heavy metal from water sample by using biomolecules and bacteria. Biosorbent have the ability to bind the heavy metal and therefore can remove from polluted water. Currently, we have taken the water sample from Ballendur Lake, Bangalore. Which is highly polluted due to industries beside this lake. This sample of water was serially diluted to 10-7. 10-4 and 10-5 diluted sample were allowed to stand in Tryptone Glucose Extract agar media mixed with the different concentrations of lead acetate for 24 hours. Microflora growth was observed. Then we cultured in different temperature, pH And different age of culture media. Finally, we did the biochemical test to identify the bacteria isolate and we found till genus level, it could be either Streptococcus sp. or Enterococcus sp.