Agroforestry plays a better role in increasing agricultural productivity by nutrient recycling, reducing soil erosion, and improving soil fertility and enhancing farm income compared with conventional crop production. Furthermore, it has promising potentials for reducing deforestation while increasing food, fodder, and fuelwood production. Agroforestry practices can reduce the atmospheric concentration of CO2 by increasing carbon storage in biomass, decreasing emission at source and modifying agricultural practices to increase the quantity of carbon stored in soil organic matter. The natural cycling of the carbon is maintained and controlled by a dynamic balance between biological and inorganic processes since the geological history of the earth. In the terrestrial ecosystem, carbon is sequestered in rocks and sediments, wetlands and forests, and in the soils of forestland, grasslands and agricultural land. In this paper, the estimation of the biomass and carbon sequestration rates of silvicultural and horticultural tree species under Horti-silviculture and Agri-Horti-Silviculture agroforestry system has been done. This investigation is proof to substantiate the fact that adoption of agroforestry techniques/systems is not only beneficial to farmers from their economic perspective but at the same time agroforestry systems helps to reduce global warming and rejuvenate the earth’s atmosphere. In four years of study carbon sequestered in the following manner: Grevillea robusta > Dalbergia sissoo > Syzygium cumini> Mangifera indica> Anacardium occidentale > Eucalyptus globules. When compared according to silvicultural and horticultural categories it Syzygium cumini was found most efficient among horticultural tree species while Grevillea robusta was most efficient among selected tree species.