Chromatography is an important technique for the separation, identification, and purification of components of a mixture for qualitative and quantitative analysis. Protein can be quantitated based on the different properties such as shape and size, total charge and binding capacity. So the different types of the method were present to quantitate the protein. Like ion exchange chromatography (IEC), size exclusion chromatography (SEC), affinity chromatography and reverse phase chromatography. Also in SEC and IEC, we compare the different types of parameters like time, resolution, cost, temperature, detectors and resolution. So comparing these methods we decide that the size exclusion chromatography method was better than the ion exchange chromatography for quantitation of protein molecules.