Glutathione is an anti-oxidant present in plants. It is used to treat lung diseases, including idiopathic pulmonary fibrosis, cystic fibrosis, and lung disease in people with HIV disease, cancer, skin pigmentation. Glutathione was also given as a shot (by injection into the muscle) for preventing poisonous side effects of cancer treatment (chemotherapy) and for treating the inability to father a child (male infertility). The Glutathione was given intravenously (by injection into the vein, by IV) for preventing “tired blood” (anemia) in kidney patients undergoing hemodialysis treatment, preventing kidney problems after heart bypass surgery, treating Parkinson’s disease, improving blood flow and decreasing clotting in individuals with “hardening of the arteries” (atherosclerosis), treating diabetes, and preventing toxic side effects of chemotherapy (cancer patient)In the following studies using bioinformatics software and tools the chemical structure of glutathione protein, drug indication, glutathione protein pathway, a gene present in the protein, binding and active site, binding interaction with other protein, domain region were predicted based on these studies it is concluded that the glutathione protein can be used to treat many human diseases.