E-Infrastructures can be defined as networked tools, data, and resources that support a community of practice (CoP), broadly including all those who participate in and benefit from research. During the UNESCO-HP BGI project (2009-2012), the University of Nigeria team conducted experiments on plant tissue culture under the theme, “sustaining the plant tissue culture component of grid computing”. Plant Tissue culture is a method for plant propagation under in vitro conditions. Different types and parts of plants (known as explants) may be cultivated in vitro. Because plant tissue culture is still in its empirical stage, it is time-consuming, cost-intensive, and manpower demanding. These necessitated the design and development of a plant tissue culture simulation application that predicts explant yields using multiple regression models. The initial version that was developed during the BGI project with a prediction accuracy of about 67%, unfortunately, was not deployable on an e-infrastructure like the grid or the cloud. Hence, during the Sci-GaIA project (2014-2017), a use case for the development of a newer version, Plantisc2, that is deployable on e-infrastructure was proposed. The outcome of which is reported in this paper.