The paper involves the development of a simple, precise and sensitive method for estimation of Apixaban in bulk drug and its marketed formulation using a reverse-phase liquid chromatographic method. The separation was achieved on C18 INERTSIL ODS-2 Column (250 mm×4.6 mm×5 um) using mobile phase (Buffer: ACN) in the ratio of 55:45(v/v) with a run time of 15 minutes and wavelength for estimation of Apixaban was taken as 280 nm. Literature survey reveals that there are very few HPLC, UV methods were available Hence an attempt has been made to develop an RP-HPLC method for estimation of Apixaban. The developed method was validated for Linearity, Accuracy (% Recovery), Precision, LOD, and LOQ etc. the linearity was found to be in the range of 1-3 μg/ml with correlation coefficient found for linearity is 0.999. The developed and validated RP-HPLC method is applied for the identification of eluted.