Development of DNA isolation and purification method with automation by using Silica coated Magnetic Nanoparticles. To develop a protocol to extract and purify the DNA, with automation using silica-coated magnetic particles, of two gram-negative bacteria E. coli DH5α for small volume culture (1ml) and large volume culture (5ml). Cell Lysis: Here all the analysis has been done on E. coli DH5α, gram negative bacteria. Washing: The second step comes in purifying the nucleic acids is the washing. Other than proteins, there are many kinds of impurities present with nucleic acids and these impurities need to be washed. Elution: The final step is the elution step. In this step, finally the DNA is extracted from the silica coated MNPs and hence purified.