In this paper, we nanomanufacture for the first time a novel DNA sequencing using nanoscale hole, in synthetic single digit nanometer thickness membrane. The DNA sequencing is carried out for each base of DNA to sequence and detect by placing a multimeter and the readings are taken on the edges of the synthetic single digit nanometer thickness membrane. The multimeter readings gives the voltage change readings for each base of DNA as there will be a change in concentration in the presence of DNA inside a nanoscale hole, in synthetic single digit nanometer thickness membrane.