Osteoarthritis (OA) of knee is most common form of arthritis and is a major cause of functional disability especially in elderly patients due to pain. Hot mustard and wax therapy are two modalities employed in management of pain. Aim: To compare the effect of hot mustard application and wax therapy on pain and disability in patients with osteoarthritis of knee. Methods and Material: The study adopts a comparative trial design. A total of sixty subjects of age 50-65 years were recruited . They were assigned to either group One ( hot mustard application) or group Two (wax therapy). Every subject were assessed for pain and disability at baseline and following ten days. Results: Pre and post data was analysed by Wilcoxon signed rank test which showed difference in VAS (P<0.00), WOMAC (P<0.00) in mustard group and VAS (P<0.00), WOMAC (P<0.00) in wax group respectively. The overall comparison between the groups was done by using Mann Whitney U test which showed decrease in VAS scale (P<0.007). Conclusions: The study result suggests that both hot mustard application and wax therapy could be considered in reducing pain