Fatigue is a common debilitating symptom which remains unrecognized among people with chronic kidney disease with significant impacts on patients’ everyday functioning. The aim of this study was to determine the effect of intradialytic aerobic exercise on fatigue in hemodialysis patients. A Quasi experimental study was carried out among 60 adult hemodialysis patients (30 each in the groups) selected by consecutive sampling technique. The baseline characteristics and pretest fatigue score were assessed for both the groups and the intradialytic aerobic exercise was administered for 8 weeks (three times/week) among the experimental group. Then post intervention I and II fatigue score were assessed in both the groups at end of 4th and 8th week. In Control group, there was no statistically significant difference in fatigue score between pre intervention, post intervention I and II (32.43 ± 7.78, 32.20 ± 7.00 and 31.13 ± 8.44, F=1.29, p = 0.28)). Whereas, in experimental group, there was significant reduction in fatigue score in post intervention I (26.97 ± 8.60) and II (23.57 ± 7.29) than pre intervention (32.20 ± 8.64) at p=0.001. There was significant difference in post intervention I (t=2.58, p= 0.001) and post intervention II (t=3.71, p = 0.001) between control and experimental group. This study shows that intradialytic aerobic exercises is beneficial in reducing fatigue, as a supplementary therapy in hemodialysis patients.