The main purpose of this study is to find out the effect of uphill training on selected physical and physiological variables among long-distance runner. For the present study, the researcher takes the male long distance runners from Pudukkottai District taken as sources of data. Thirty male long distance runners, who had participated in intercollegiate tournaments, were preferred as an area under discussion for this study. The age of the subject matter was ranging from 18 years to 25 years. The criterion measures adopted for the study measuring the physical and physiological variables are given below. To measure the physical variables 600 m run for speed endurance, 12 minutes run and walk for cardiorespiratory endurance, pulse count for resting pulse rate and Harvard step test for vo₂ max the effect of uphill training on selected physical variables and physiological variables among long-distance runners the independent “t” test was used at 0.05 level of significance. Result: There was a significant difference in experimental groups in speed endurance, Cardiorespiratory endurance, resting pulse rate and vo₂ max.