Introduction: Health care workers are at a greater risk of contact with infectious diseases such as Ebola virus disease the worst hit in 2014 and may promote transmission by occupational exposures. Objectives: To evaluate the effectiveness of Integrated Pathway on knowledge and attitude of nurses regarding care of people affected with EVD. Methodology: Quasi Experimental research approach, Time Series Design Non Equivalent Control Group Design was adopted. In the experimental and control group, each comprising 16 study participants, totally 32 from two different tertiary care hospitals, at Coimbatore. Knowledge and attitude questionnaire were used and analyzed by using descriptive statistics. Results: There was the highest significant difference between pre test and post test 3 score of knowledge of nurses in care of people affected with EVD (t = 21.2354; p < 0.05), whereas the lowest level of significant difference was found between post test 1 and post test 3 score of knowledge of nurses in care of people affected with EVD (t = 3.4802 ; p < 0.05). Also in control group, it depicted that there was significant difference found between pre test & post test 1 score of knowledge of nurses in care of people affected with EVD (t = 1.7111 ; p < 0.05), whereas the lowest level of significant difference was noted between post test 2 and post test 3 score of knowledge of nurses in care of people affected with EVD (t = 0.2559 ; p < 0.05). Conclusion: The study had proved the necessity to create a great impact on the level of knowledge and attitude of nurses.