Removal nutrients by utilization from the culture medium and accumulations of lipid by oleaginous microorganisms are the promising route for the biodiesel feedstock production. Most of the oleaginous microbes cannot directly utilize starch, some of them modify the nutrient resources like cellulose and starch into monosaccharides initially, and then these fermentable sugars will be converted into fatty acids. In the present work, we investigated how the co-culturing of Aspergillus tamarii and Chlamydomonas reinhardtii involves in nutrient removal, starch-digestion, and lipid deposition when cultivated in potato processing wastewater. We found that combined cultivation and consolidated bioprocessing the fungi and algae stimulate nutrient removal, starch-digestion, and biodiesel feedstock production effectively. The results exhibited the highest biomass (18.5g/L and 19.3g/L) with the lipid content of 43% and 57% on 10 and 25% diluted potato processing wastewater after the 4th day of incubation. Maximum depletion of Ammonium nitrogen (34 mg/L) Orthophosphates (14 mg/L) and starch-digestion was 89% was obtained at 25% diluted potato processing wastewater after the 4th day of incubation. Considering the yield, lipids derived from starch using Aspergillus tamarii and Chlamydomonas reinhardtii would be a promising alternative source for biodiesel feedstock production.