Ulcer is the most common prevalent gastro intestinal disorder, which affects approximately 10 -15% of people in the world. It makes major global health problem today. Ulcer is a open sore, it can be developed inside the inner lining of the stomach (gastric ulcer) or the small intestine (duodenal ulcer). Both the ulcers are also commonly referred as peptic ulcers. Objectives: The present study was carried out to investigate anti-ulcer activity of Ethanol extract of Indigofera tinctoria on albino rats. Materials and Methods: The present study was carried by pylorus ligation induced ulcer models in albino rats. The antiulcer activity of ethanol extracts of Indigofera tinctoria (125, 250 mg/kg p.o. 7 days) was compared with standard drugs (Famotidine). In pyloric ligation induced ulcer model, the studied parameters were gastric volume, pH, total acidity, ulcer score, and ulcer index. Results: In pyloric ligation model; the volume of gastric content, total acidity, ulcer score were significantly decreased at p<0.01 and pH of the gastric juice was significantly increased at p<0.05 in EEIT treated groups as compared to control group. All the doses of EEIT showed dose dependent antiulcer effect as well as significant (p<0.01) reduction in the ulcer index as compared to control group in all the experimental models. The ethanol extract of EEIT at 250 mg/kg has more potent antiulcer activity than 125 mg/kg of ethanol extract of EEIT. Conclusion: The results of the study indicate that the EEIT has better potential against ulcer which supports the traditional claims in folklore medicine.