Electron Paramagnetic Resonance (EPR) spectroscopy is a spectroscopic technique that can be used to study complexes containing unpaired electrons, due to a phenomenon known as the Zeeman effect. This paper will aim at performing an experiment to retrieve and analyze Electron Paramagnetic Resonance (EPR) Spectra. Samples of 2,2-diphenyl-1-picrylhydrazyl (DPPH), Myoglobin, and Manganese Chloride were analyzed through room temperature and low-temperature EPR runs, using liquid nitrogen as the cooling agent. The spectra obtained were then used to calculate quantitative values and the values were analyzed and interpreted to provide the nature of the unpaired electrons in the complex. Lastly, the results were compared with literature values and future opportunities for research about EPR in biochemistry and geology were also discussed.