Abstract Solanum nigrum L. (Kaambal) (Kashmiri) has been traditionally used to treat pathological ailments like fever, ulcers, bacterial infections, fungal infections, jaundice and liver disorders (Creasy et al.,1981; Capizzi et al., 2003). Column chromatography in chemistry is a method used to purify individual chemical compound from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process.The classical preparative chromatography column is a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter (a glass frit or glass wool plug – to prevent the loss of the stationary phase) at the bottom.