Background: Cardiovascular ailments belong to some of the most important contributing factors for the mortality and reduction in quality of life in developing nations. Patients on prescriptions with many medications are at a heightened risk for the development of adverse reactions especially resulting from drug-drug interactions. This study aimed at assessing the pattern, proportion and impact of such Drug-Drug Interactions (DDIs) in patients with various cardiovascular and non-cardiovascular co-morbidities. Methods: This study was conducted in the Department of Internal Medicine of a tertiary care center. The prescription of 200 patients was analysed for demographic information like gender, age, comorbidities and drugs prescribed. DDI was assessed using Micromedex software. Results: This study included 200 prescriptions of people aged 60 years and above. A total of 324 DDIs were identified among which, 24 (7.4%)were major and 127 (39.2)were moderate. The antiplatelet and anticoagulant group of drugs were responsible for the majority of DDI, followed by hypertension medications and diabetic medications Conclusions: It can be concluded from this study that the incidence of DDI rises with the rise in the number of medications in the prescriptions and there is an increase in the number of drugs in the prescription with the increase in the number of co-morbidities.