In the present research, the synthesis of silver nanoparticles by the green method is done using stem and leaves aqueous extract of Tinospora Cordifolia (T.C). The pathway of nanoparticles formation is by means of reduction of silver nitrate by extracts, which act as both reducing and capping agents. The silver nanoparticles characterized by UV-Vis-spectrometer, Fourier transform infrared spectroscopy, X-ray diffractometer, Scanning electron microscopy, Energy dispersive spectroscopy. The sizes of the synthesized silver nanoparticles are found to be in the range of 27- 58 nm. The energy dispersive spectrum confirmed the presence of silver metal. The silver nanoparticles synthesized in this process have the efficient antimicrobial activity against pathogenic bacteria like Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Staphylococcus aureus and Serratia marcescens using paper disc diffusion method.