The present experiment is to study the effect of Tulasi, kusha, and copper on the Physical properties of water. In the study, commonly growing Tulasi (basil), and dry kusha, and the commercially available copper wire were used. Tulasi, kusha, and copper with a weight of 100 mg, 250 mg, and 500 mg were used. Materials were immersed in the glasses with 100 ml water and conductivity, turbidity, and pH of the water was measured at the time duration of immediate, two hours, four hours, six hours, and 24 hr. The Experiment conducted for five trials with three replications each and a total of 15 numbers. Kruskal-Wallis test was applied to assess changes between groups in all variables. Tulasi and copper decrease conductivity and turbidity of water irrespective of their weight while there was an exponential significant increase of these properties of water by kusha with the direct relation of its weight during all the duration of time. Tulasi, kusha, and copper decrease the pH of water for all the duration of time, and this decreasing trend is more by increasing their weight. Tulasi and copper decrease conductivity and turbidity of water while kusha increases these properties. All these materials decrease the pH of water during all the duration of time and this decreasing trend is more by increasing their weight.