Job Satisfaction is conscious willfulness to seek for other alternatives in other organizations. Human resource management (HRM) is known as the central business concern, that shapes the behavior, attitudes, and performance of the employees, This study empirically evaluated six Human Resource (HR) practices (Careful recruitment and selection, Extensive remuneration systems, Team working and flexible job design, Training and learning, Employee involvement, Performance appraisals with links to contingent reward systems) and impact on the Job Satisfaction of the employees in five-star hotels in Sri Lanka. The sample consisted of one hundred and twenty-six employees working in all five-star hotels in Sri Lanka. The data were gathered by administering questionnaires. Considering the relationship between Human resources practices and Job Satisfaction, the analysis represents that there is a positive and the relationship between Careful recruitment and selection, Extensive remuneration systems, Team working and flexible job design, Training, and learning, Employee involvement, Performance appraisals with links to contingent reward systems. The result was implying that the effect of Team working and flexible job design on Job Satisfaction is higher than in other relationships. However, Human resource practices play a vital role in Job Satisfaction in accordance with all level employees in five-star hotels. Further Team working and flexible job design is more important to employee Job satisfaction.