Erythrina Variegata plant as revealed from various literature resources, our work will be planned to predict the bioactivity of Erythrina Variegata leaves extracts using the ‘PASS’ software. The PASS predictions for the bioactivity of the sixteen flavonoids were found to be in the ranges from 65 to 97.7% as various inhibitors. Among the sixteen flavonoids from Erythrina Variegata leave, Vogel – A and Isowigteone were found to exhibit more bioactivity like Monophenol monooxygenase inhibitor (96.3, 97.7%), Membrane integrity agonist (91.4, 92.6%), CYP1A inhibitor (95.2, 95.3%), CYP1A1 inhibitor (95.7%), MMP9 expression inhibitor (89.8, 90.6%), Histidine kinase inhibitor (93.7, 94.4%), UGT1A10 substrate (92.4, 95.3%), Chalcone isomerase inhibitor (93.1, 93.0%), Aldehyde oxidase inhibitor (91.3, 89.4%), CYP1A substrate (89.1%).