The growth medium is the sole source of nourishment for the bacteria cultured under laboratory conditions. This implies that the growth medium could largely influence the quality and quantity of secondary metabolites produced by the bacterium. Secondary metabolites are the weapons used by bacteria for survival in competitive ecosystems. In this regard, Siderophores are among the vital secondary metabolites of marine bacteria which help them in sourcing the minimal iron essential for their growth and virulence from the marine environment. Among the 11 different growth media evaluated for their impact on Siderophore production by the emerging marine pathogen Vibrio campbellii, MM9 native (minimal salt medium) was found to yield the maximum siderophore units (95.35%). The key factors influencing the growth of V. campbellii were found to be glucose and casamino acid. The determinant factors influencing Siderophore production were found to be glucose and iron-depleted composition of MM9 native medium.