The present investigation deals with the high-frequency callus induction studies on Abrus precatorius L., It belonging to family Fabaceae, an economically important multipurpose plant. The plant is scared of novel antidiabetic properties. The plant shows poor seed germination capacity (30%) due to hard seed coat and conventional vegetative regeneration methods are a complete failure. Therefore, the propagation of this plant by tissue culture techniques is an urgent need and well justified. Nodal intermodal and tendril explant were used for the callus induction studies. Axillary shoot proliferation on Murashige and Skoog’s (MS) basal medium fortified with 2, 4 di chlorophenoxyacetic acid (2, 4-D) and Kinetin (Kin). In combination with auxins at different concentrations. The best callus induction was obtained with MS basal medium contain (2, 4-D 1.5 mg/l + Kin 0.5 mg/l) for node explants. Like in MS medium where (75%) of the internode responded with (BAP 3 mg/l +kin 0.5 mg/l+ NAA 0.5 mg/l), at same time responsible with tendril leaf (84%) (2, 4-D 2.0 mg/l + Kin 1.0 mg/l). The results will facilitate the conservation and propagation of this important medicinal plant.