Cheilanthes farinosa is a pteridophytic plant belongs to the family Pteridaceae is a rare plant of rhizome up to 1 cm. in diam. Sori minute, 1 mm. in diam, closely set along the margins of the pinna segments. The plant roots are used to treat eczema and stomachache; fronds are used to treat menstrual disorders. Rhizomes and roots are used to treat gout, rheumatism and used as general tonics. It is used in the treatment of inflammatory, skin disorders and rheumatism etc., So the Cheilanthes farinosa plant was chosen for conservation through micropropagation. The sporangia are used as explant. MS medium with 3.5mgL-1 of BAP and GA3 shows the high growth rate and 2.4mgL-1 of IBA and KIN shows the slow growth rate. After 1 month of inoculation, the gametophytic body was developed. And it was subculture to MS medium containing various concentrations of BAP and GA3.