Information about the normal values of interpupilary distance (IPD) for particular age group, gender and ethnic background has important clinical implications. Normative values of IPD could be useful in the diagnosis of certain syndromes and also in surgery after facial trauma. Our main aim to carrying out this study was to find out the effect of gender and age on interpupilary distance in total study sample of 30 subjects within the age limit of 15 to 60 years and IPD measurements were compared taken with two different instruments i.e. PD ruler and auto refractrometer. Data was collected using descriptive cross sectional study at Ophthalmology Department at Medina Teaching Hospital, Faisalabad. Proper room for examination with all instruments that were used in study was chosen after history taking. With torch light we examined that the pupil is round, regular and reactive. Then IPD values were measured using a millimeter PD ruler and auto refractrometer. Our study results show that gender has an effect on IPD measurements and also that males have 3mm wider papillary distance than females. Our study subjects were divided into 3 age groups and IPD in younger age (15-30 years) group keep on increasing, after wards it remains constant. And another objective of our study was to compare IPD values with different instruments i.e. PD ruler and auto refractrometer used and results are significant. It is suggested that IPD should be an integral part of eye examination to avoid any future intolerance of spectacles and asthenopic symptoms.