Streptococcus and Micrococcus luteus are the best source of antibiotic. In the present study 4 culture (Bacillus cereus(S6), Pseudomonas fluoresens(S1), Streptococcus(S2), Micrococcus luteus(S3) were isolated from 6 soil sample collected from Lucknow, Kanpur and Unnao. Isolation of microbes was done by serial dilution method. Primary screening of the culture was done by streaking. Gram’s staining was done to check the culture was pure or not. Identification was done by Bergey’s manual, further media optimization was done. Production media was prepared for these 4 culture in which the best carbon sources was Sucrose, Glucose, Dextrose, Sucrose best nitrogen sources were Yeast extract, the best temperature was 370C and best pH was 11,7,7,7(Micrococcus luteus, Streptococcus, Pseudomonas fluorescens, Bacillus cereus) respectively. Further extraction of seconda ry metabolite was done by using one solvent :(Chloroform) for extracellular component and (Methanol) for the intracellular component. Further purification of the antimicrobial compound using TLC (thin layer chromatography) and a spectrophotometer was performed resulting Quercetin compound.