This paper evaluates the knowledge and practice of dengue fever infection among nursing staff in Bin Nasir hospital Saudia Arabia in 20017 82 nurses enrolled in the study. A descriptive study design – Was adopted for this study 82 staff nurses selected randomly,grouping ter age from 20 up to 41 and with different education level and different work experience and departments, self administered questionaired filled regard knowledge and practice, data arranged tabulated analysed (descriptive and inferential statistics) using spss 20.results respondents revealed good knowledge towards dengue fever, their mean knowledge 63.4%, p .000 and good practice, mean score 64.6% p .000, the results showed significant association between qualification, work experience and work department with both knowledge and practice Ps are .055, .023, .500. conclusion respondent showed good knowledge and practice, also showed the highly significant association between qualification, work experience and work area and both knowledge and practice.recommendation.stablish of regular session towards knowledge and prevention toward dengue fever.