Technology has brought massive evolution in the application development process. The trend of online business has enhanced interest in migrating organizations towards modern technologies. Service providers, vendors, and clients are worried about security when valuable credentials of the organizations are placed online over the web. Therefore, security protocols and cryptographic techniques are used to prevent vulnerability. Several authentication techniques have been used to secure information, this article describes some of them. The concepts from three famous techniques were adopted to practice and propose a novel authentication method MO-Auth. Experimental work was conducted by implementing two web-based applications on which MO-Auth was tested. To validate results ISO/IEC 9126 quality characteristics were evaluated for each authentication method including MO-Auth. By considering ISO/IEC 9126 quality frameworks, we have found MO-Auth as easy to use, reliable, efficient, less cost, easy to maintain and much secure authentication method. Moreover, we have also conducted a survey-based on available features in modern applications, which indicates that usage of traditional authentication techniques is not decreased but still being used with the collaboration of modern technologies, while methods like OAuth and Biometrics are newer but their usage will be increased in future.