Acinetobacter baumanii is considered to be one of the significant pathogens causing nosocomial infections and outbreaks in hospital settings, especially in ICU. The dreadful concern with Acinetobacter infections is their ability to gain resistance against all commonly used antibiotics like Penicillin, cephalosporins, Fluoroquinolones, carboxypenicillin, carbapenems as a result this Multi-Drug Resistant (MDR) strains increases patient hospital stay, cost of treatment, mortality, and morbidity among affected patients. A total of 146 Acinetobacter isolates were received from various clinical samples. Samples received were routinely subjected to Culture. Gram staining was done. The smear showed Gram-Negative Coccobacilli taken and subjected for further biochemical reactions. Antibiotic susceptibility testing was done by Kirby Bauer disc diffusion method in Muller -Hinton agar plate. The majority of Acinetobacter baumanii isolates were received from the 19-40 age group (45.8%), followed by the 41- 60 (26% ) age group. Most of the Acinetobacter baumanii strains were isolated from wound swab 33.5%, followed by urine 29.4%, sputum 17.1%, Blood 10.2%.Antibiotic sensitivity of 146 Acinetobacter baumanii isolates showed Highest resistance to Ampicillin (100%), Gentamycin (70.5%),Cotrimoxazole (68.4%) , Piperacillin Tazobactam (66.4%) , Ciprofloxacin (63%), Nitrofurantoin (61.6%) , Ceftriaxone (61.6%). Levofloxacin, Amikacin, Tigecycline, Colistin were found to be the most effective antibiotics with a sensitivity of 73.2%, 87.6%, 91.7%, 100% respectively. Of 146 isolates 63 (43%) were Multi-Drug Resistant, i.e isolates showing resistance to more than 3 classes of antibiotics. Hence strict infection control and antimicrobial stewardship policies should be implemented in hospitals to reduce mortality and morbidity associated with Acinetobacter baumanii infections.