The present study was conducted to develop Nano Nano Vitamin D3 chewable tablet. The experiment was designed accordingly ZEON F&D SOP for the Nano Vitamin D3 chewable tablet. ZLL/F&D/024. Five formulations were carried for development of Nano Vitamin D3 to check the overall acceptability. For the development of Nano Vitamin D3, experimental design with the active ingredient (Nano Vitamin D3 powder), lubricant, glidant, diluents, and flavor produced different combinations that were studied using pre and post evaluation parameters. The methodology of Nano Vitamin D3 chewable tablets was carried out by dry mixing method. The chewable tablet was compressed by using tablet compression machine. A Nano Vitamin D3 chewable tablet was orange granular powder. Nano Vitamin D3 was the main factor of the chewable tablet, Nano increases the bioavailability of Vitamin D3 which posses significant role in the various disease like Osteoporosis, Muscle weakness, Hypertension, Multiple sclerosis, Malabsorption, Rickets, Cancer, Rheumatoid Arthritis(RA), Diabetes, Tuberculosis (TB) etc.