There is an information gap in the knowledge about fishes consumed in Lagos state especially as it relates to the nutritional values of these fish species. In this study, the nutritional evaluation of selected fish specimens Croaker (Pseudotolithus elongates), Tilapia (Oreochromis aureus), and Crab (Callinectes sapidus) were carried out. Samples were purchased from selected markets in Ikorodu, Badagry, and Lagos Island, all in Lagos State. Proximate analysis (Protein, Fat, and Ash) was determined. Protein determination was done using the micro Kjeldahl method with the mean values ranging between 19.90±0.34% and 20.60±0.78% for Pseudotolithus elongatus, 29.40±0.4% and 30.52±0.5% for Callinectes sapidus, 18.50±0.6% and 20.80±0.00% Oreochromis aureus. Ash content was carried out and the mean values ranged from 1.20±0.10% and 1.13±0.10% for Pseudotolithus elongates, 1.20±0.00% and 1.30±0.00% for Oreochromis aureus, from 8.90±0.10% and 10.10± 0.20% for Callinectes sapidus. Crude fat was carried out using the soxhlet extraction method and the values determined gravimetrically and were found to range between 1.39±0.00% and 1.50±0.02%. for Pseudotolithus elongates, 1.10±0.00% and1.3±0.10% for Oreochromis aureus, 0.5±0.04% and 0.9±0.03% for Callinectes sapidus. Vitamins A, D, B3, B6, and B 12 were determined using the HPLC.