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A review on molecular methods in the detection of foodborne pathogens

Food safety is a major healthcare concern globally. Foodborne pathogens are held responsible for the elevated incidence of foodborne diseases worldwide, which also has an economic impact more so in developing countries. For these reasons, it is crucial to supply food that has undergone testing and is safe for consumption. Initially, testing for pathogens solely relied on culture-based methods; which turned out to be time-dependent and labor-intensive. Identification of pathogens in a short time with standard accuracy stood as a challenge. After decades of research, rapid detection methods were developed to ensure food safety at an industrial level. Rapid techniques comprised of nucleic acid-based methods like PCR, multiplex PCR, nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), and DNA microarray; immunological-based methods like enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassays and optical, electrochemical, and mass-related biosensor were the popularly used methods. DNA markers were also commercially available for the detection of the target organism in the sample. The main purpose to devise these molecular techniques was to provide rapid, highly sensitive, and specific results while being economically convenient. This report summarizes the existing molecular methods and improvements that were made over the years for the detection of foodborne pathogens.

Published by: Asmita Pramanic

Author: Asmita Pramanic

Paper ID: V7I2-1497

Paper Status: published

Published: April 26, 2021

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Research Paper

Static structural analysis of split muff coupling under maximum load conditions

The split muff coupling is also called compression coupling. It is a rigid coupling, the main advantage of this coupling is that it runs at a heavy load with a moderate speed. In the current paper, static structural for the coupling is done using Ansys Workbench 2019 and the CAD model is created using Solidwork. This paper deals with Static Structural analysis of Split Muff Coupling under maximum torsional load condition. Static Structural is much needed and monitoring that for maximum loading conditions for Split Muff Coupling approach can be used for prophecy purpose.

Published by: Prajwal Sudhir Kulkarni

Author: Prajwal Sudhir Kulkarni

Paper ID: V7I2-1466

Paper Status: published

Published: April 26, 2021

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Research Paper

Structural analysis of spanner bolt assembly

A spanner or span is a metallic tool whose end fits round a nut or bolt so that you can turn it to loosen or tighten it. Spanner is designed to grip a nut or bolt head. In this paper, Open Ended Spanner is taken into consideration for the study of structural analysis. The open-ended spanner has a U-Shaped that grips the two opposite faces of a hexagonal bolt or nut. The main aim of this paper is to evaluate the structural analysis of the Spanner Bolt Assembly. Input will be the Spanner Bolt Assembly CAD model and output will be deformation and stress plots. The CAD model and Analysis operations are performed using Catia and Ansys Workbench respectively. Structural analysis is performed on Spanner Bolt Assembly with Structural Steel material to validate Equivalent stress and Total Deformation.

Published by: Ashutosh Rajput, Tanmay Deshpande

Author: Ashutosh Rajput

Paper ID: V7I2-1417

Paper Status: published

Published: April 26, 2021

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Others

Assessment of deterioration in RCC building

Concrete is considered a durable material but it is still potentially vulnerable to deterioration unless certain precautions are taken. The assessment of concrete structures consists of not only an evaluation of the present condition but also a prediction of the cause of deterioration and its residual life. A number of tests need to be carried out to assess the extent of distress and to estimate the quality of concrete, before taking up any repair measures. This paper discusses the assessment of RCC building by using Non-Destructive Testing. The main aim of the paper is to highlight the importance and significance of different test methods employed to assess the present condition of the RCC structure.

Published by: Mubassar Zamindar, P. A. Dode

Author: Mubassar Zamindar

Paper ID: V7I2-1367

Paper Status: published

Published: April 26, 2021

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Research Paper

Drainage system analysis of Gunjavani river basin, Velhe, Pune district

Morphometry is the precise and objective measurement of landforms. Morphometry involves quantitative analysis of geometric properties of the future Strahler. A.N. (1957) describes the term morphometry measurement of the shape or geometry of any natural form. While Clarke J.I. (1970) defines morphometry as the measurement and mathematical analysis of the configuration of the earth’s surface and of shape, and dimensions of the land forces.

Published by: Sachin Kantilal Pise

Author: Sachin Kantilal Pise

Paper ID: V7I2-1458

Paper Status: published

Published: April 24, 2021

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Research Paper

Lung disease detection using deep learning

The motivation of the project is to detect five to seven lung diseases using chest x-rays. Lung diseases include Asthma, tuberculosis, pneumonia, bronchitis, Chronic Obstructive Pulmonary Disease (COPD). In addition to it, in recent days, we are all scared of the word “corona”. Though there is the screen of blood and mucus for the test, the major test to confirm covid positive is – X-RAY. A further developed version of this model will help us in the diagnosis of covid from viral_pneumonia. The model involves two modules 1. segmentation - where FC descent 103 is used to get the segmented masks of lung and 2. resnet algorithm is used to predict the class of the diseases based on probability voting based on the k patches acquired in every image .saliency map used in it indicates the region where the lung areas are mostly affected.

Published by: Kavinkumar Kaliyannan, Rithika Sivamani, Sheikh Tajamul

Author: Kavinkumar Kaliyannan

Paper ID: V7I2-1467

Paper Status: published

Published: April 24, 2021

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