There is no plant in the world that is non-medicinal, or which cannot be used as medicine is used as medicine. Folk or traditional systems of medicines always played an imperative role in the global healthcare system. Antidesma alexiteria L (Thalimaram), belongs to the Phyllanthaceae family. Antidesma species has been used in many traditional medicines to treat diarrhea, skin complaints, hemorrhages, and abdominal disorders. The present study focuses on the preliminary Phytochemical screening and evaluation of the antioxidant potential of Antidesma alexiteria L. For qualitative analysis, standard tests were used to identify phytochemicals present in the extract and confirmed the presence of carbohydrates, coumarins, glycosides, saponins, phenols, and phytosterols. Leaf extracts were also subjected to total phenolic content(TPC), total tannin content (TTC), and total flavonoid content(TFC). In quantitative estimation, the TPC content of each extract was 24.13, 32.65 & 3.89 mg of GA/g extract, TFC was 230.38, 493.85 &217.15 mg of Ru/g extract and total tannin were 613, 503 &39.67 mg of CTN/g extract respectively. Antioxidant properties of the three extracts were evaluated using DPPH assay, Nitric oxide scavenging activity, and total antioxidant activity. Ascorbic acid was used as standard. In DPPH free radical scavenging assay IC50 value of alcoholic, hydroalcoholic, and aqueous root extracts were found to be 650.7,534.1 and 7704.18μg/mL respectively. For the Nitric Oxide Radical Scavenging assay IC50 value of alcoholic, hydroalcoholic, and aqueous root extracts was found to be 928.9, 354.6, and 380.24 μg/mL respectively. The total antioxidant activity of the plant extracts was calculated from the calibration curve of ascorbic acid, and the results showed 49.76, 44.97 and84.14 mg of AA/g activity for ethanolic, hydroethanolic, and aqueous extracts respectively